Type of I.P :
Patent A government authority or licence conferring a right or title for a set period, especially the sole right to exclude others from making, using, or selling an invention.
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Others Trade Secerts, Innovative Step, Creative etc
Patent Title : A Method for Purification of Intracellular Protein
Patent Registration No : PI20094405
R & D
R&D The invention is at the lab stage or proof concept stage
Pre-Commercial The invention is currently in the process of field testing
Ready For Market Invention is ready to be licensed out or sold
Selling Method :
Exclusive Licensing No person or company other than the named licensee can exploit the relevant intellectual property rights. Importantly, the licensor is also excluded from exploiting the intellectual property rights.
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Assignment The sale of ownership to the individual assigning the intellectual property.
Product Year :
The present specification discloses an integrated purification technique for purifying recombinant intracellular protein (GFP) from intact E. coli cells using preparative PAGE column. Under the influence of electric fields, the negatively charged GFP was drifted out from cells without the need of cell disruption process.
The electrophoresed cells were observed under SEM and some on the membrane of cells were observed. GFP was then separated from other host cells host protein along poluacrylamide gel and finally eluted electrophoretically from the gel.
The invention has successfully recovered the GFP with purity and yeild 95% and 82%, respectively in 100 min operation.
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